ABSTRACT
Objective: To study the chemical constituents of saponins in the stems and leaves of Panax ginseng. Methods: The chemical constituents were isolated and purified by various chromatographic methods, and their structures were identified by NMR and MS data analysis. Results: Nine compounds were isolated and identified as 3β,6α,12β,25-tetrahydroxy-dammar-E-20(22)-ene-6-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside (1), sanchinoside B1 (2), 3β,6α,12β-dammar-E-20(22)-ene-3,6,12,25-tetraol (3), ginsenoside Rk3 (4), ginsenoside Rh4 (5), notoginsenoside T2 (6), 3β,6α,12β-dammar-20(21),24-diene-3,6,12-triol (7), ginsenoside Rk1 (8), and ginsenoside Rg5 (9). Conclusion: Compound 1 is a new natural product and the other eight compounds are all isolated from the stems and leaves of P. ginseng for the first time.
ABSTRACT
Objective: To study the chemical constituents in the acid hydrolysates of total saponins from the stems and leaves of Panax ginseng. Methods: The chemical constituents were isolated and purified by various chromatographic methods, and their structures were identified by NMR and MS data analysis. Results: Thirty-four compounds were isolated and identified as 3β-acetoxy-12β-hydroxy-20(R), 25-epoxydammarane (1), 3β, 6α-diacetoxy-12β-hydroxy-20(R), 25-epoxydammarane (2), 3β-acetoxy-6α, 12β-dihydroxy-20(R), 25-epoxydammarane (3), 20(R)-panaxadiol (4), isodehydroprotopanaxatriol (5), 3-oxo-6α, 12β-dihydroxy-20(R), 25-epoxydammarane (6), dammar-(E)-20(22)-ene-3β, 12β, 25-triol (7), 6α-acetoxy-3β, 12β-dihydroxy-20(R), 25-epoxydammarane (8), 20(S)-protopanaxadiol (9), 20(R)-protopanaxadiol (10), 20(S)-25-ethoxyl-dammarane-3β, 12β, 20-triol (11), 20(R)-panaxatriol (12), dammar-(E)-20(22), 24-diene-3β, 6α, 12β-triol (13), 27-demethyl-(E, E)-20(22), 23-dien-3β, 12β-dihydroxydammar-25-one (14), 3β, 12β-dihydroxy-22, 23, 24, 25, 26, 27-hexanordamaran-20-one (15), 3β-acetoxy-6α, 12β, 25-trihydroxy-20(S), 24(R)-epoxy-dammarane (16), 20(S)-25-ethoxyl-dammarane-3β, 12β, 20-triol (17), dammar-(E)-20(22)-ene-3β, 6α, 12β, 25-tetrol (18), dammar-(Z)-20(22)-ene-3β, 6α, 12β, 25-tetrol (19), 20(S)-dammarane-3β, 12β, 20, 25-tetrol (20), 20(R)-dammarane-3β, 12β, 20, 25-tetrol (21), 20(S)-protopanaxatriol (22), 20(R)-protopanaxatriol (23), (20S, 24S)-dammarane-20, 24-epoxy-3β, 6α, 12β, 25-tetraol (24), (20S, 24R)-dammarane-20, 24-epoxy-3β, 6α, 12β, 25-tetraol (25), (20R, 24R)-dammarane-20, 24-epoxy-3β, 6α, 12β, 25-tetraol (26), 3β, 6α, 12β-trihydroxy-22, 23, 24, 25, 26, 27-hexanordamaran-20-one (27), 12β-hydroxy-20(R), 25-epoxydammarane-3β-O-β-D-glucopyranoside (28), 20(S)-dammarane-3β, 6α, 12β, 20, 25-pentol (29), 20(R)-dammarane-3β, 6α, 12β, 20, 25-pentol (30), 20(R)-dammarane-3β, 6α, 12β-trihydroxy-20, 25-epoxy-6-O-β-D-glucopyranoside (31), pseudo-ginsenoside-Rh2 (32), 20(S)-ginsenoside-Rh1 (33), and 20(R)-ginsenoside-Rh1 (34). Conclusion: Compounds 1-3, 6, 8, 11, 17, 24-26, and 32 are ginseng triterpenoids isolated from the acid hydrolysates of total saponins from the stems and leaves of P. ginseng for the first time. Pseudo-ginsenoside-Rh2 is a potent antiproliferative agent against human cancer cells.
ABSTRACT
Objective: To study the chemical constituents of saponins in the stems and leaves of Panax ginseng. Methods: The chemical constituents were isolated and purified by various chromatographic methods, and the chemical structures were identified by NMR and MS data analyses. Results: A total of 39 compounds were isolated and identified. Among them, 17 compounds were determined as ginsenoside Re (1), 20(S)-ginsenoside Rh1 (2), 20(R)-ginsenoside Rh1 (3), ginsenoside Rh5 (4), 20(E)-ginsenoside F4 (5), ginsenoside F2 (6), 20(S)-ginsenoside Rg3 (7), 20(R)-ginsenoside Rg3 (8), 20(S)-ginsenoside Rf2 (9), 20(R)-ginsenoside Rf2 (10), 20(S)- protopanaxadiol (11), 20(R)-protopanaxadiol (12), 20(S)-ginsenoside Rh2 (13), 20(R)-ginsenoside Rh2 (14), 20(S)-protopanaxatriol (15), 20(R)-protopanaxatriol (16), and ginsenoside Rd (17). Conclusion: Compound 9 is a new saponin. Compounds 2-10, 13, and 14 are rare ginsenosides.